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Can structural insights to ligase system and substrate specificity be exploited to produce novel catalysts, kinds that could support in comprehending the genetics and cell biology of DNA repair and while in the observe of enzymatic joining and synthesis of nucleic acids? There is nice motive to Consider so. Nature has presently confounded the partitioning of ligases into rigid DNA-precise and RNA-unique clades. We now recognize Ligaz Slots that lots of DNA ligases are adept at sealing substrates made up of an all-RNA 3′-OH strand (8, 25, 26) Which some RNA ligases commonly seal molecules with as couple as two or a few ribonucleotides on the three′-OH terminus in an or else all-DNA context (27, 28).
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